Purification and characterization of an antibacterial protein from haemolymph of Sarcophaga peregrina (flesh-fly) larvae
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چکیده
منابع مشابه
Mode of action of a bactericidal protein induced in the haemolymph of Sarcophaga peregrina (flesh-fly) larvae.
The mode of action of a bactericidal protein (sarcotoxin I) purified from the haemolymph of Sarcophaga peregrina (flesh-fly) larvae was studied, focusing attention on its effect on the function of the membrane of Escherichia coli. Sarcotoxin I almost completely blocked the uptakes of tetraphenylphosphonium ion and proline, which are known to be driven by a membrane potential, indicating that th...
متن کاملCharacterization of the antimicrobial peptide derived from sapecin B, an antibacterial protein of Sarcophaga peregrina (flesh fly).
Sapecin B, an antibacterial protein of Sarcophaga peregrina, was divided into four peptides. A hendecapeptide derived from its helix region was found to have comparable antibacterial activity with that of the complete protein. This peptide had a much wider spectrum of antimicrobial activity than that of sapecin B, exhibiting activity on not only Staphylococcus aureus (Gram-positive) and Escheri...
متن کاملA novel protease in the pupal yellow body of Sarcophaga peregrina (flesh fly). Its purification and cDNA cloning.
We purified a novel serine protease with a molecular mass of 26 kDa from Sarcophaga pupae. This protease appeared almost exclusively in the yellow body, an organ that develops temporarily in the pupae of dipteran insects and expands to form the adult midgut by engulfing the larval midgut. cDNA analysis revealed that this protease consists of 239 amino acid residues and has significant structura...
متن کاملPurification and Characterization of an Inhibitor of the Cysteine Protease from the Hemolymph of Sarcophaga peregrina
The hemolymph of Sarcophaga peregrina (flesh fly) larvae was found to contain multiple inhibitors of hemocyte cysteine protease. One of them, named sarcocystatin A, was purified and found to be a mixture of the components sarcocystatin A, and AP in a molar ratio of 2:l. These components can exist in either the associated or dissociated form. The apparent heterogeneity of the protease inhibitors...
متن کاملisolation and purification of an antibacterial protein from immune induced haemolymph of american cockroach, periplaneta americana
background: antimicrobial peptides play a role as effectors substances in the immunity of vertebrate and invertebrate hosts. in the current study, antimicrobial peptide was isolated from the haemolymph of the american cockroach, periplaneta americana . methods: micrococcus luteus as gram-positive bacteria and escherichia coli as gram-negative bacteria were candidate for injection. induction ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1983
ISSN: 0264-6021
DOI: 10.1042/bj2110727